Introduction-
- Enzymes are work as a biological catalysts that increase the rate of reaction(biochemical reaction).
- Mainly enzymes are in 3D structure globular proteins(tertiary and quaternary structure)
- Enzyme are macromolecular biological catalysts, enzyme accelerate or catalyze chemical reaction.
- During the reaction substrate bind with the enzyme at active site of enzyme , converts into different molecule called products.
- microbial enzyme are the biological catalysts for the biolchemical reaction leading to microbial growth and respiration ,as well as formation of fermentation products.
Structure of enzyme-
The active site of an enzyme is a region that binds substrates ,co-factors and prosthetic groups. Active site is less than 5% of the total surface area of enzyme.active site have tertiary structure of proteins.
active site further divided into two types
Binding site- it choose the substrate and binds to active sites
Catalytic site- it performs the catalytic action of enzyme
Characteristics;
- Enzyme increase the rate of reaction
- The presence of enzyme does not effects the nature and properties of end products
- Small amount of enzyme accelerate the chemical reaction
- Enzyme are sensitive to change in pH, temp, and substrate concentration\.
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